Anti-EGFP antibody [F56-6A1.2.3] ab184601 from Abcam

Product Specs
ItemAnti-EGFP antibody [F56-6A1.2.3]
CompanyAbcam
Price Supplier Page View Company Product Page
Catalog Numberab184601
Quantity100 UG
ApplicationsImmunocytochemistry/ Immunofluorescence, Western blot
Conjugate/TagUnconjugated
Formatin vitro produced
IsotypeIgG2b
CloneF56-6A1.2.3
HostMouse, Nude mouse
Antibody TypePrimary
ClonalityMonoclonal
Concentration1 mg/ml
ReactivitySpecies independent
TargetEGFP

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Abcam

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Cambridge Biomedical Campus

Cambridge CB2 0AX

United Kingdom

Phone: +44 (0) 1223 696000 (option 1)
+44 (0) 1223 696000 (option 2)
+44 (0) 1223 771600

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Website: http://www.abcam.com

(1) FOXI3 pathogenic variants cause one form of craniofacial microsomiaNature CommunicationsApril 11, 2023Ke Mao, Christelle Borel, Muhammad Ansar, Angad Jolly, Periklis Makrythanasis, Christine Froehlich, Justyna Iwaszkiewicz, Bingqing Wang, Xiaopeng Xu, Qiang Li, et al.chemiluminescence reagent (WBKLS0100, Millipore, Billerica, MA, USA). The primary antibodies is anti-EGFP (ab184601, Abcam, Cambridge, UK; 1:1000 dilution), and secondary antibody is Goat ani mouse lgG(H + L)-HRP (P03S01L,

(2) TDP-43 pathology and functional deficits in wild-type and ALS/FTD mutant cyclin F mouse modelsNeuropathology and applied neurobiologyApril 1, 2023van Hummel A, Sabale M, Przybyla M, van der Hoven J, Chan G, Feiten AF, Chung RS, Ittner LM, Ke YD.were used: pan‐TDP‐43 (10782‐2‐AP, Proteintech), POU3F2 (ab94977, Abcam), GFAP (G9269, Sigma), EGFP (ab184601, Abcam), Cyclin F (sc‐515207, Santa Cruz) and VCP (sc‐20799, Santa Cruz). Microscopy was performed with

(3) Convection-enhanced delivery of nanoencapsulated gene locoregionally yielding ErbB2/Her2-specific CAR-macrophages for brainstem glioma immunotherapyJournal of NanobiotechnologyFebruary 20, 2023Lin Gao, Chongdeng Shi, Zhenmei Yang, Weiqiang Jing, Maosen Han, Jing Zhang, Cai Zhang, Chunwei Tang, Yuanmin Dong, Ying Liu, et al.EGFP expression was analyzed by western blotting. A monoclonal antibody against EGFP (Abcam, cat. no. ab184601) was used at a dilution of 1:1000, and goat anti-mouse IgG-HRP (1:5000, Abcam, cat. no. ab6789) was

(4) Loss of small GTPase Rab7 activation in prion infection negatively affects a feedback loop regulating neuronal cholesterol metabolismJournal of Biological ChemistryFebruary 1, 2023Pearl Cherry, Li Lu, Su Yeon Shim, Vincent Ebacher, Waqas Tahir, Hermann M. Schatzl, Samia Hannaoui, Sabine Gilchpolyclonal anti-Lamp1 (ab24170), mouse monoclonal anti-Rab7 (ab50533), and mouse monoclonal anti-EGFP (ab184601) antibodies were procured from Abcam. Rabbit monoclonal anti-Rab5 (C8B1) and anti-Rab6 (D37C7) antibodies

(5) Efficient Modulation of Exon Skipping via Antisense Circular RNAsResearch (Wash D C)January 19, 2023Shuaiwei Ren, Mei Huang, Raoxian Bai, Lijiao Chen, Jiao Yang, Junyu Zhang, Wenting Guo, Weizhi Ji, Yongchang Chenroom temperature. The blocked membrane was incubated with dystrophin (Abcam #ab154277), EGFP (Abcam #ab184601), or vinculin (Cell Signaling Technology (CST) #4650S) overnight at 4 °C and then incubated with anti-rabbit

(6) CENP-F-dependent DRP1 function regulates APC/C activity during oocyte meiosis INature communicationsDecember 13, 2022Zhou CJ, Wang XY, Dong YH, Wang DH, Han Z, Zhang XJ, Sun QY, Carroll J, Liang CG.Control, Abcam ab6046; 1:500), mouse anti-cMyc (Thermo Fisher R950-25; 1:400), mouse anti-EGFP (Abcam ab184601; 1:400), rabbit anti-cyclinB1 (Cell Signaling 4138; 1:200), goat anti-cyclinB2 (R&D Systems AF6004;

(7) Trypsin is a coordinate regulator of N and P nutrients in marine phytoplanktonNature CommunicationsDecember 1, 2022Yanchun You, Xueqiong Sun, Minglei Ma, Jiamin He, Ling Li, Felipe Wendt Porto, Senjie Linconfirmed at a protein level through Western blot using anti-EGFP antibody. The anti-EGFP (Cat no. ab184601) and anti-GAPDH (Cat no. ab59164) antibodies used in this study were purchased from Abcam (England),

(8) Hepatocyte phosphatase DUSP22 mitigates NASH-HCC progression by targeting FAKNature communicationsOctober 8, 2022Ge C, Tan J, Dai X, Kuang Q, Zhong S, Lai L, Yi C, Sun Y, Luo J, Zhang C, et al.anti-Flag (#ab205606), anti-CD11b (#ab133357), anti-DUSP22 (#ab70124), anti-DUSP3 (#ab248113), anti-EGFP (#ab184601), anti-HNF4a (#ab201460), anti-CK19 (#ab52625) and anti-α-SMA (#ab124964) were purchased from Abcam

(9) Transmembrane domain of IFITM3 is responsible for its interaction with influenza virus HA(2) subunitVirologica SinicaOctober 1, 2022Xu W, Wang Y, Li L, Qu X, Liu Q, Li T, Wu S, Liao M, Jin N, Du S, et al.(A/Shanghai/1/2013) (Immune Technology, Cat# IT-003-0073M1, USA), Anti-EGFP antibody (F56-6A1.2.3) (Abcam, Cat# ab184601, UK), Anti-mCherry antibody (Abcam, Cat# ab167453, UK), GAPDH (14C10) Rabbit mAb (Cell Signaling Technology,

(10) Combining protein and RNA quantification to evaluate promoter activity by using dual-color fluorescent reporting systemsBioscience ReportsSeptember 30, 2021Yan Peng, Xin Huang, Tianfang Huang, Feng Du, Xin Cui, Zhuo Tangwere rabbit monoclonal to α-tubulin (ab179484, Abcam, Cambridge, U.K.), rabbit monoclonal to EGFP (ab184601, Abcam, Cambridge, U.K.). Proteins recognized by the antibodies were detected by ImageQuant LAS 500

(11) Recognition of lipoproteins by scavenger receptor class A membersJournal of Biological ChemistryAugust 1, 2021Chen Cheng, Enlin Zheng, Bowen Yu, Ze Zhang, Yuanyuan Wang, Yingbin Liu, Yongning HeCaCl2 or 1 mM EDTA, 0.1% Triton X-100, pH 7.4) and then incubated with the mouse anti-GFP antibody (ab184601, Abcam) for 1 h. After washing with the washing buffer (25 mM Hepes, 150 mM NaCl, 10 mM CaCl2 or 1 mM

(12) Internalization of HMGB1 (High Mobility Group Box 1) Promotes Angiogenesis in Endothelial CellsArteriosclerosis, thrombosis, and vascular biologyDecember 1, 2020Lan J, Luo H, Wu R, Wang J, Zhou B, Zhang Y, Jiang Y, Xu J.fluoride) membranes. After blocking, the blots were incubated with an anti-EGFP antibody (1 μg/mL, ab184601; Abcam) or polyclonal antibody against β-actin (0.2 μg/mL, sc-1615; Santa Cruz Biotechnology, TX). The

(13) Novel enhanced GFP-positive congenic inbred strain establishment and application of tumor-bearing nude mouse modelCancer scienceOctober 1, 2020Lan Q, Chen Y, Dai C, Li S, Fei X, Dong J, Shen Y, Dai X, Lu Z, Liu B, et al.EGFP, Nestin, GFAP, Ki67 and FAP followed the manufacturer’s instructions. Primary antibodies EGFP (ab184601, Abcam), Nestin (ab6142, Abcam), FAP (fibroblast activation protein, ab53066, Abcam), Ki67 (14‐5698‐82,

(14) Promoter choice: Who should drive the CAR in T cells?PloS oneJanuary 1, 2020Rad S M AH, Poudel A, Tan GMY, McLellan AD.using RIPA lysis buffer and blotting carried out using mouse monoclonal anti-EGFP antibody (Abcam, #ab184601), rabbit anti-human Mcl-1 (Abcam, #ab28147), biotin anti-c-myc (Biolegend #908805). Mouse monoclonal

(15) An efficient protein production system via gene amplification on a human artificial chromosome and the chromosome transfer to CHO cellsScientific reportsNovember 18, 2019Ohira T, Miyauchi K, Uno N, Shimizu N, Kazuki Y, Oshimura M, Kugoh H.described previously43. Membranes were blotted with rabbit monoclonal antibody against EGFP antigen (ab184601, 1:2,000, Abcam, Cambridge, UK) or with polyclonal antibody against α-tubulin (PM054–7, 1:5,000; MBL,

(16) The human ABCB6 protein is the functional homologue of HMT-1 proteins mediating cadmium detoxificationCellular and Molecular Life SciencesOctober 1, 2019Zsófia Rakvács et al.used in Western blotting experiments: β-actin (A1978, Sigma-Aldrich, Saint Louis, MO, USA); anti-EGFP (ab184601 Abcam, Cambridge, UK), ABCB6-567 [11], anti-HA antibody, (H6908 Sigma-Aldrich). The HRP-dependent luminescence

(17) Microtubule‑severing protein Katanin p60 ATPase‑containing subunit A‑like 1 is involved in pole‑based spindle organization during mouse oocyte meiosisMolecular Medicine ReportsAugust 22, 2019Lei‑Lei Gao, Fei Xu, Zhen Jin, Xiao‑Yan Ying, Jin‑Wei Liu15‑234) was purchased from Antibodies Incorporated. Mouse monoclonal anti‑EGF P (F56 ‑ 6A1.2.3) (cat. no. ab184601) was purchased from Abcam. Cy2‑conjugated donkey anti‑mouse IgG (code no. 715‑225‑150), rhodamine (TRITC)‑conjugated

(18) Prognostic implications and interaction of L1 methylation and p53 expression statuses in advanced gastric cancerClinical epigeneticsMay 14, 2019Shin YJ, Kim Y, Wen X, Cho NY, Lee S, Kim WH, Kang GH.Santa Cruz Biotechnology, CA, USA), β-actin (1:1000, sc-47,778, Santa Cruz Biotechnology), and EGFP (1:1000, ab184601, Abcam, Cambridge, UK). After three 15-min washes with Tris-buffered saline containing 0.1% Tween 20,

(19) The hippocampal extracellular matrix regulates pain and memory after injuryMolecular psychiatryDecember 1, 2018Tajerian M, Hung V, Nguyen H, Lee G, Joubert LM, Malkovskiy AV, Zou B, Xie S, Huang TT, Clark JD.#B-1355; Vector labs, USA), mouse monoclonal anti-GFP (anti-green fluorescent protein; 1:200, cat. #ab184601; Abcam, USA), and rabbit polyclonal anti-Aggrecan (1:500, cat. #AB1031; Millipore, USA). The following

(20) Clusterin protects neurons against intracellular proteotoxicityActa neuropathologica communicationsNovember 7, 2017Gregory JM, Whiten DR, Brown RA, Barros TP, Kumita JR, Yerbury JJ, Satapathy S, McDade K, Smith C, Luheshi LM, et al.

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Anti-EGFP antibody [F56-6A1.2.3] from Abcam

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Anti-EGFP antibody [F56-6A1.2.3] from Abcam

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